Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7
نویسندگان
چکیده
منابع مشابه
Stx2-specific human monoclonal antibodies protect mice against lethal infection with Escherichia coli expressing Stx2 variants.
Shiga toxin-producing Escherichia coli (STEC) strains are responsible for causing hemolytic-uremic syndrome (HUS), and systemic administration of Shiga toxin (Stx)-specific human monoclonal antibodies (HuMAbs) is considered a promising approach for prevention or treatment of the disease in children. The goal of the present study was to investigate the ability of Stx2-specific HuMAbs to protect ...
متن کاملExtraintestinal pathogenic Escherichia coli carrying the Shiga Toxin gene stx2.
The 2011 outbreak of infections with Escherichia coli with characteristics of both enteroaggregative E. coli (EAEC) and Shiga toxin-producing E. coli (STEC) caused a paradigm shift with regard to the human pathogenicity of STEC (1). Diarrheagenic E. coli (DEC) usually does not cause extraintestinal diseases such as urinary tract infections and bacteremia (2). The DNA of 193 E. coli isolates fro...
متن کاملStructure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7.
Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1. To investigate the differences among the Stxs, which are al...
متن کاملNatural killer T (NKT) cells accelerate Shiga toxin type 2 (Stx2) pathology in mice
Shiga toxin-producing Escherichia coli (STEC) is a leading cause of childhood renal disease Hemolytic Uremic Syndrome (HUS). The involvement of renal cytokines and chemokines is suspected to play a critical role in disease progression. In current article, we tested the hypothesis that NKT cells are involved in Stx2-induced pathology in vivo. To address this hypothesis we compared Stx2 toxicity ...
متن کاملRelationship between stx genotype and Stx2 expression level in Shiga toxin-producing Escherichia coli O157 strains.
To determine the expression level of Shiga toxin (Stx) 2-related toxins (Stx2 and Stx2c) produced by each of 33 Stx-producing Escherichia coli (STEC) O157 strains, stx2 and stx2c mRNAs (stx2-related mRNA) were measured using real-time PCR with primers that recognize sequences common to stx2 and stx2c. The amount of Stx2 and Stx2c protein was measured using a reversed passive latex agglutina...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2010
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2010.04.099